Journal: Brain and behavior
Article Title: SP140 inhibitor suppressing TRIM22 expression regulates glioma progress through PI3K/AKT signaling pathway.
doi: 10.1002/brb3.3465
Figure Lengend Snippet: FIGURE 6 SP140 inhibitor suppress glioma progress and TRIM22/PI3K/AKT signaling pathway. (A) Coexpression analysis of TRIM22 and SP family in TCGA glioma database. (B) The mRNA and protein expression level of TRIM22 in different cell lines. (C) The mRNA and protein expression level of TRIM22 in U87 and U251 after GSK761 intervention. (D) CCK-8 analysis showed cell proliferation in U87 and U251 after GSK761 intervention. (E, F) Wound-healing assay showed cell migration in U87 and U251 after GSK761 intervention. (G, H) Transwell invasion assay showed cell invasion in U87 and U251 after GSK761 intervention. (I) The mRNA expression level of TRIM22 after GSK761 treatment. (J) Western Blot analysis showed PI3K/AKT signaling pathway changes after GSK761 intervention. *p < .05, **p < .01, ns indicates no significance.
Article Snippet: The primary antibodies used in this study were anti-PI3K antibody (67121-1-Ig, Proteintech), anti-AKT antibody (60203-2-Ig, Proteintech), anti-pho-AKT antibody (80455-1-RR, Proteintech), anti-TRIM22 antibody (ab68071, Abcam).
Techniques: Expressing, CCK-8 Assay, Wound Healing Assay, Migration, Transwell Invasion Assay, Western Blot